Insights into the history of a bacterial group II intron remnant from the genomes of the nitrogen-fixing symbionts Sinorhizobium meliloti and Sinorhizobium medicae
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چکیده
منابع مشابه
Genetic relationship of Sinorhizobium meliloti and Sinorhizobium medicae strains isolated from Caucasian region.
Sinorhizobium meliloti and Sinorhizobium medicae are two closely related species of the genus Sinorhizobium showing a similar host range, nodulating leguminous species of the genera Medicago, Melilotus and Trigonella, but their phylogenic relationship has not been elucidated yet. In this paper we report the application of three different molecular markers, (i) RFLP of nodD genes, (ii) 16S-23S r...
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Use of the computer-retargeted group II intron RmInt1 of Sinorhizobium meliloti for gene targeting
Gene-targeting vectors derived from mobile group II introns capable of forming a ribonucleoprotein (RNP) complex containing excised intron lariat RNA and an intron-encoded protein (IEP) with reverse transcriptase (RT), maturase, and endonuclease (En) activities have been described. RmInt1 is an efficient mobile group II intron with an IEP lacking the En domain. We performed a comprehensive stud...
متن کاملSplicing of the Sinorhizobium meliloti RmInt1 group II intron provides evidence of retroelement behavior
Group II introns act as both large catalytic RNAs and mobile retroelements. They are found in organelle and bacterial genomes and are spliced via a lariat intermediate, in a mechanism similar to that of spliceosomal introns. However, their distribution and insertion patterns, particularly for bacterial group II introns, suggest that they function and behave more like retroelements than organell...
متن کاملExcision of the Sinorhizobium meliloti group II intron RmInt1 as circles in vivo.
Excision of group II introns as circles has been described only for a few eukaryotic introns and little is known about the mechanisms involved, the relevance or consequences of the process. We report that splicing of the bacterial group II intron RmInt1 in vivo leads to the formation of both intron lariat and intron RNA circles. We determined that besides being required for the intron splicing ...
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ژورنال
عنوان ژورنال: Heredity
سال: 2014
ISSN: 0018-067X,1365-2540
DOI: 10.1038/hdy.2014.32